Description
When a beam of light passes through a light-absorbing substance (usually a solution), the solute absorbs light energy and the intensity of the light decreases. Absorbance is a physical quantity used to measure the degree of light absorption. Based on the absorbance measurement is simple and easy to implement and easy to use, absorbance measurement is widely used in liquid and gas spectral measurement technology, it can also integrate the application into the industrial application environment and customers concerned about the test. Absorbance spectroscopy can quantitatively identify substances, or it can quantitatively analyze the concentration of molecules in a solution.
Compared with the traditional experiment to determine the absorbance, the spectrometer test is simple and efficient, and the experimental results are accurate and reliable, which frees scientific researchers from complicated experimental operations and reduces human errors. The spectrometer is based on a modular design, and you can change the equipment layout according to changing experimental needs; replaceable equipment layout, continuously upgraded experimental operations and applications make it possible for a varietyof experimental applications now and in the future.
Principle
The direct relationship between the absorbance and concentration of a solution is called Lambert-beer's lawThe spectrometer can be used to test several sets of solution samples with known molar concentration to calculate the matching curve between the solution absorbance and concentration, so as to measure the solution samples with unknown concentration.
Features
• No darkroom operation, simple operation, low reagent consumption, good repeatability, high measurement accuracy and fast detection
• Absorbance value can be read in real time, data can be repeatedly imported, read and used
Application
• Liquid absorbance measurement
• Solution concentration measurement
Parts List
Wavelength range | UV-VIS | VIS-NIR |
Spectrometer | 200-900nm,resolution ~1nm | 400-1100nm,resolution ~1nm |
Light Source | BIM-6213,Mini-Deuterium Tungsten light source | |
Fiber | SIM-6102-0605,Quartz fiber need 2 pcs | |
Cuvette Holder | BIM-6305-01,Universal cuvette holder | |
Cuvette | SIM-6301-Q10,Quartz cuvette, 10mm optical path | |
Software | BSV spectral analysis software |
*For detailed parameters of each part, please click the link in the table
Measurement case: KMnO4 solution
Absorbance curves of KMnO4 solutions with different concentrations
Establish concentration data model to measure KMnO4 solution of unknown concentration